FastQ Format

https://en.wikipedia.org/wiki/FASTQ_format

@FREDDYKRUEGER:1:1:6:417/1
GGAGCGATTTGTCTGGTTAATNCCGTNAACNAACTAGNCCTCANNNNNNNNNNNANNNANNNNNNNNNNNNNNNNN
+FREDDYKRUEGER:1:1:6:417/1
aa_S^OY]^\SV_WSOS\_]YD[`WTDZa[DX_BBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBB
@FREDDYKRUEGER:1:1:6:2017/1
CTTCAATGTATTCTGGAGGATNCTTANGCCNGAATTTNTGATTNNNNNNNNNNNGNNNCNNNNNNNNNNNNNNNNN
+FREDDYKRUEGER:1:1:6:2017/1
abbbaa`_^a`_a`]\_[[]WD^__^DVa\DT^a^BBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBB
@FREDDYKRUEGER:1:1:6:1825/1
GGAGAAACCAATATCTCTTAANCCTGNGCANATCCGGNATGAGNNNNNNNNNNNGNNNCANNNNNNNNNNNNNNNN
+FREDDYKRUEGER:1:1:6:1825/1
abbaabbaaa^]a`aaa`^a^D^`]SDX`^D^^a`BBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBB
@FREDDYKRUEGER:1:1:6:1315/1
AAATACCAAAGATCAAGGAAGNAAGANTGANGAAGATNTAAGGNNNNNNNNNNNANNNGANNNNNNNNNNNNNNNN
+FREDDYKRUEGER:1:1:6:1315/1
aabbababaa]aXaaa\Ub_WD[a\^DL^[DO`aBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBB
@FREDDYKRUEGER:1:1:6:2027/1
CATTAATCAAGAACGAAAGTTNGGGGNTCGNAGACGANCAGATNNNNNNNNNNNCNNNACNNNNNNNNNNNNNNNN
+FREDDYKRUEGER:1:1:6:2027/1
abbbbb`aa`V_a`Y_aa]ZXDTMVMDU`WD[R_BBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBB
@FREDDYKRUEGER:1:1:6:1669/1
GGGCCGTGTCTCAGTCCCAGTNTGGCNGATCATCCGAAAAGACNANCNNNNNNNCNTNGGCTNNNNNNNCNNNTNN
+FREDDYKRUEGER:1:1:6:1669/1
````b`^Xaa`b`YYbbb``WDTVTYDT^`a``a`X`^BBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBB
@FREDDYKRUEGER:1:1:6:1269/1
CAGAAGTTGAATCCAACCTTGNAAGGGACTGTTCATAAGGCAGNANCNNNNNCNCNTNGGATGTNNNGAAATNAGN
+FREDDYKRUEGER:1:1:6:1269/1
aa`a_Zaa_abbbba`abb_TD^_YZ_V``\_bbbbb_YBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBB

Applications and Tools that use it:

1. Applications that asses the quality of the sequence.

   1. fastqc

2. Input and output format for tools that remove and trim low quality sequences

   1. fastx-toolkit, trimmomatic

3. Sequence aligners

   1. bowtie, bwa, etc.

4. Assemblers

   1. abyss, velvet, allpaths, trinity, etc.